A simple, rapid and precise reverse phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for simultaneous estimation of moxifloxacin and metronidazole in bulk drug and in a synthetic mixture. The analysis was carried out using Hypersil ODS C18 (250 mm x 4.6 mm, 5m Make: thermo scientific) pre packed column. The separation was carried out using a mobile phase containing a buffer and acetonitrile taken in the ratio (45:55 v/v), was pumped at a flow rate of 0.8 ml/min with UV-detection at 314 nm. Both the drugs were well resolved on the stationary phase and the retention times were around 2.174 minutes for moxifloxacin and 3.467 minutes for metronidazole. The method was validated and shown to be linear for moxifloxacin and metronidazole. The correlation coefficients for moxifloxacin and metronidazole are 0.9997 and 0.9992 respectively.
Loading....